ANTT minimises the risk of contamination transfer to critical surfaces. It should be used during vaccine preparation and assembly.

Implementing aseptic non-touch technique

Aseptic non-touch technique (ANTT) minimises the risk of contamination transfer to critical surfaces during manipulations. It is achieved by disinfecting sites and ensuring equipment is removed from protective packaging immediately before use.

In all cases operators should sanitise their hands prior to commencing vaccine preparation and follow local guidance around use of gloves.

Using trays for assembly of consumables

If a tray is used as the preparation work area it should be disinfected on all sides with alcohol wipes before starting preparation.

If trays are only being used to hold the vials and equipment before preparation there is no need to disinfect the tray surface. In this case, the bench area should be disinfected.

Disinfecting vial bungs and ampoule necks with alcohol wipes

Vial bungs and ampoule necks should be disinfected and allowed to dry before the bung is punctured or the ampoule opened.

Handling syringes and needles

The outer packaging does not require disinfection if preparation is in a clinical area.  The packaging should be opened immediately before the connection between needle and syringe is made.

  1. Open the needle packaging by peeling the packaging apart to only expose the needle connection. Do not remove the needle from the packaging and place on the surface as this risks contaminating the hub.
  2. Open the syringe packaging by peeling apart and removing the syringe. Avoid touching the syringe tip.
  3. Connect the syringe and needle immediately before placing in the work area.
  4. Only remove needle sheath immediately before use for vaccine reconstitution/dose withdrawal.

Use in conjunction with other material

This page should be read in conjunction with other material on vaccine preparation, in particular our Standard Operating Procedures.

Information and guidance specific to COVID-19 Vaccine AstraZeneca

Resources covering pharmaceutical aspects of the vaccination programme specific to COVID-19 Vaccine AstraZeneca

Information and guidance specific to COVID-19 Vaccine Pfizer-BioNTech

Resources covering pharmaceutical aspects of the vaccination programme specific to COVID-19 Vaccine Pfizer-BioNTech

Information and guidance specific to COVID-19 Vaccine Moderna

Resources covering pharmaceutical aspects of the vaccination programme specific to COVID-19 Vaccine Moderna

COVID-19 Vaccine Pfizer-BioNTech Handling and Preparation Video

A video produced by Pfizer showing the correct handling and preparation of the COVID-19 Vaccine Pfizer-BioNTech